De Sciglio, 25, picked up his injury on Sunday during pre-match preparations for his team's 2-1 Serie A victory against Sassuolo.
"Having pulled up in the warm-up of Sunday's win over Sassuolo, Mattia De Sciglio underwent tests, which revealed a tear to the flexor muscles in his right thigh," the Italian club said in a statement.
"The defender has started to receive therapy and is undergoing a personalized recovery program, as his condition continues to be monitored on a daily basis," the club added.
The Juventus squad - minus De Sciglio - exercised at the team's training center in Turin before heading to Spain, where head coach Massimiliano Allegri has scheduled a press conference for Tuesday evening.
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