Otamendi and Mercado, who started in Thursday's 0-3 defeat to Croatia, suffered blows to their right ankle, reports Efe.
Biglia, who was a substitute against Croatia, was suffering from knee discomfort in Friday training, according to Argentina's medical staff.
The three players worked out in the gym, as coach Jorge Sampaoli began preparations for Tuesday's game against Nigeria.
After suffering a 1-1 draw and 0-3 defeat against Iceland and Croatia respectively, Argentina, which is at the bottom of Group D with a single point, needs to win to qualify for the next round of last 16 of the 2018 World Cup in Russia.
(This story has not been edited by Social News XYZ staff and is auto-generated from a syndicated feed.)
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