Monaco midfielder Moutinho caught a flu, while Borussia Dortmund defender Guerreiro has sustained a leg injury, reports Efe.
Portugal star Cristiano Ronaldo, defenders Ruben Dias, Pepe and Cedric Soares and midfielders Manuel Fernandes and Goncalo Guedes took part in the session led by head coach Fernando Santos.
Portugal drew 3-3 against Spain in their opening match last Friday and won 1-0 over Morocco in the Group B second match on Wednesday.
They are set to play Iran at Mordovia Arena in the Russian city of Saransk on Monday.
Portugal and Spain lead Group B with four points each, followed by Iran with three and Morocco with zero points so far.
(This story has not been edited by Social News XYZ staff and is auto-generated from a syndicated feed.)
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