Neymar, who was having difficulty kicking the ball, left the group training accompanied by Brazil physiotherapist Rodrigo Mazziotti on Tuesday, reports Efe.
Minutes later, Brazil released a statement from team doctor Rodrigo Lasmar saying that Neymar had pains in the right ankle caused by a foul received in Sunday's 1-1 draw with Switzerland.
Neymar will train normally with the rest of the group on Wednesday, according to the statement.
Neymar underwent an operation on his right foot on March 3 and while he recovered in record time, the superstar has yet to regain his best form.
Brazil is second in Group E behind Serbia, who defeated Costa Rica 1-0 on Sunday.
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